Violet Red Bile Lactose Agar (VRBL) (ISO), 500g
Brand: Neogen® Culture Media
Analyte: Coliforms, E. coli, Enterobacteriaceae
Platform: Dehydrated Culture Media
Mass: 500 g
Grams per Litre: 38.50
Litres per 500 g: 13.00
Plates per 500 g: 710.00
No supplements required
A medium for the enumeration of coliform organisms in food and dairy products according to ISO 4832:2006 and is not intended for use in the diagnosis of disease or other conditions in humans. The selectivity of the medium is due to the presence of bile salts and crystal violet. Lactose fermenters produce red or purple colonies, often surrounded by a halo of the same color. Non-lactose fermenters produce pale colonies. Selectivity can be increased by incubation at 42–44 °C.
Typical Formulation
Formula Litre
Yeast Extract 3.0 g/L
Enzymatic Digest of Animal Tissue 7.0 g/L
Sodium Chloride 5.0 g/L
Bile Salts 1.5 g/L
Lactose 10.0 g/L
Neutral Red 0.03 g/L
Crystal Violet 0.002 g/L
Agar 12.0 g/L
Preparation
Suspend 38.5g of the medium in one liter of purified water.
Heat with frequent agitation and boil for one minute to completely dissolve the medium.
DO NOT AUTOCLAVE.
Cool to 45–50 °C.
MAECENAS IACULIS
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ADIPISCING CONVALLIS BULUM
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