Tryptone Soy Broth (TSB), 500g

Tryptone Soy Broth (TSB) is a general-purpose broth that will support the growth of a wide range of microorganisms and is not intended for use in the diagnosis of disease or other conditions in humans.

Tryptic Soy Broth, a general-purpose medium, is commonly referred to as Casein Soya Bean or Soybean-Casein Digest Broth and abbreviated as TSB. This medium was originally developed for use without blood in determining the effectiveness of sulfonamides against pneumococci and other organisms. Clostridia and non-sporulating anaerobes grow luxuriantly in this broth when incubated under anaerobic conditions. TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry.

Enzymatic Digest of Casein and Enzymatic Digest of Soybean Meal are nitrogen sources in TSB. Dextrose is the carbon energy source that facilitates organism growth. Sodium Chloride maintains osmotic balance; Dipotassium Phosphate is a buffering agent.

For Casein Soya Bean Digest Broth QC tested according to the USP/EP/JP methodology and requirements, please use NCM0004.

Typical Formulation

Formula Litre
Enzymatic Digest of Casein 17.0 g/L
Enzymatic Digest of Soybean 3.0 g/L
Sodium Chloride 5.0 g/L
Dipotassium Hydrogen Phosphate 2.5 g/L
Glucose Monohydrate 2.5 g/L

Preparation

Suspend 30 grams of the medium in one litre of purified water.
Mix thoroughly.
Autoclave at 121ºC for 15 minutes.