Sabouraud Dextrose Agar, 500g

Sabouraud Dextrose Agar is used for the cultivation of fungi. Conforms to Harmonized USP/EP/JP performance requirements and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized Pharmacopeia for the isolation and identification of Candida albicans from non-sterile samples. Conforms to USP/EP/JP performance specification. The medium is commonly abbreviated to SDA. The medium is also used for the preparation and maintenance of fungal test strains as described by the Harmonized Pharmacopeia. The peptone digests and dextrose provide a nutritious base for luxuriant fungal growth and the acidic pH affords selectivity against bacteria. Due to the high carbohydrate content and low pH this medium is highly sensitive to overheating which will cause a drop in the gel strength. According to the Harmonized Pharmacopeia, Sabouraud Dextrose Broth is used as an enrichment broth, with subculture performed onto Sabouraud Dextrose Agar.

Typical Formulation

Formula Litre
Dextrose 40.0 g/L
Peptic Digest of Animal Tissue 5.0 g/L
Pancreatic Digest of Casein 5.0 g/L
Agar 15.0 g/L

Preparation

Suspend 65 grams of the medium in one liter of purified water.
Heat with frequent agitation and boil for one minute to completely dissolve the medium.
Autoclave at 121°C for 15 minutes.
Cool to 45–50 °C.