Plate Count Agar (Standard Methods), 500g

Plate Count Agar (Standard Methods Agar) is used for the enumeration of bacteria in water, wastewater, food, and dairy products in a laboratory setting. Plate Count Agar is not intended for use in the diagnosis of disease or other conditions in humans. This formula conforms to ISO 4833-1&2:2013, ISO 17410:2001, American Public Health Association (APHA), Association of Official Analytical Chemists (AOAC) and FDA Bacteriological Analytical Manual. Plate Count Agar (Standard Methods Agar) is free from selective supplements and relatively rich in nutrients, making it ideal for the enumeration of viable organisms, either following a pour plate method, or a surface plate method which can be coupled with a spiral plater. Plate Count Agar (Standard Methods Agar) is also referred to as Tryptone Glucose Yeast Agar. This formula is specified in standard method procedures.

Typical Formulation

Formula Litre
Enzymatic Digest of Casein 5.0 g/L
Yeast Extract 2.5 g/L
Dextrose (Glucose) 1.0 g/L
Agar 15.0 g/L*
* 9–18 g according to gel strength

Preparation

Suspend 23.5 g of the medium in one liter of purified water.
Heat with frequent agitation and boil for one minute to completely dissolve the medium.
Autoclave at 121°C for 15 minutes.
Cool to 45–50 °C.