Mueller Hinton Agar I, 500g

Mueller Hinton Agar I is used for antimicrobial sensitivity testing by the disc diffusion method and is not intended for use in the diagnosis of disease or other conditions in humans.

A medium for antimicrobial sensitivity testing by the disc diffusion method. This medium, used in the technique of Bauer and Kirby, has been adopted by the Clinical and Laboratory Standard Institute (CLSI), formerly the National Committee for Clinical Laboratory Standards (NCCLS), in the USA as the definitive method for susceptibility testing. The medium has a very low thymine and thymidine content, making it suitable for trimethoprim and sulphonamide testing, controlled to ensure correct zone sizes with aminoglycoside and tetracyline antibiotics. The medium was originally formulated as a heat-labile, protein-free medium for the isolation of pathogenic Neisseriaceae.

Typical Formulation

Formula Litre
Beef Extract 2.0 g/L
Acid Hydrolysed Casein 17.5 g/L
Starch 1.5 g/L
Agar
17.0 g/L

Preparation

Suspend 38 grams of powder in one liter of purified water.
Heat with frequent agitation and boil for one minute to completely dissolve the medium.
Autoclave at 121°C for 15 minutes.
Cool to 45–50 °C.