EconoFit Nuvia IMAC Column, Ni-charged, 1x1ml

EconoFit Nuvia IMAC Ni-Charged Columns are convenient, disposable, prepacked low-pressure chromatography columns. EconoFit Columns offer both increased run-to-run uniformity and high purity of protein through the column design and novel resin technology. Compatible with the aqueous buffers most commonly used for protein purification, EconoFit Columns offer improved performance for your protein separation needs.

Immobilized metal affinity chromatography (IMAC) is an excellent chromatography technique for the purification of histidine-tagged proteins. The principle of IMAC is based on the affinity histidine has for metal ions. Side chains on the nitrilotriacetic acid (NTA) functional ligand selectively bind recombinant histidine-tagged proteins when the resin is charged with Ni2+ or other metals. The advantage of this technique is that proteins can often be purified close to homogeneity in a single step.

These columns are packed with Bio-Rad’s specially designed Nuvia IMAC Ni-Charged Resin. Such characteristics as the polymeric nature, optimized ligand density, and open pore structure of the Nuvia IMAC Bead result in superb mechanical strength with high stringency, low nonspecific binding, and the ability to perform separations at high flow rates.

EconoFit Columns are disposable, easy-to-use, prepacked chromatographic columns supplied ready for use in convenient 1 and 5 ml sizes. They are quickly connected to liquid chromatography systems using 10-32 fittings. Columns are available for a variety of chromatographic techniques, including desalting (size exclusion [SEC]), ion exchange (IEX), affinity (AC), mixed-mode, and hydrophobic interaction chromatography (HIC).