ddPCR Microsatellite Instability (MSI) Kit
The ddPCR Microsatellite Instability (MSI) Kit provides sensitive, reproducible, and qualitative results for the detection of deletions and insertions in five mononucleotide microsatellite markers in plasma or tumor tissue samples.
The ddPCR Microsatellite Instability (MSI) Kit includes:
- ddPCR Multiplex Supermix (2 aliquots)
- ddPCR MSI Assay 1 (BAT25/BAT26)
- ddPCR MSI Assay 2 (NR21/NR24)
- ddPCR MSI Assay 3 (Mono27)
- ddPCR Positive Control
- Uracil-DNA Glycosylase (UDG)
- Nuclease-Free Water (2 aliquots)
The ddPCR Microsatellite Instability (MSI) Kit provides sensitive, reproducible, and qualitative results for the detection of deletions and insertions in five mononucleotide microsatellite markers in plasma or tumor tissue samples.
Further, the kit allows for the detection of deletions and insertions in microsatellite markers BAT25, BAT26, NR21, NR24, and Mono27 in plasma or tumor tissue, including formalin-fixed paraffin-embedded (FFPE) tissue, from colorectal cancer (CRC) patients and other potentially MSI-relevant cancers. The kit consists of three assays: Assay 1 (BAT25 and BAT26), Assay 2 (NR21 and NR24), and Assay 3 (Mono27), as well as positive and no template controls and enough reagents for 200 reactions. Additionally, an automated software tool is available for sample analysis.
Features and Benefits
- Evaluate MSI markers with both FFPE and plasma samples
- No need for normal tissue samples
- Improved workflow and cost efficiency over current methods
- Automated data analysis with user-defined parameters
- Excellent concordance with gold standard IHC and PCR-CE methods
Applications and Uses
Detecting microsatellite instability in tissue and plasma samples from colorectal cancer patients and other potentially MSI-relevant cancers.
Note: For research use only.
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