Cetrimide Agar, 500g

Cetrimide Agar is used in the isolation and identification of Pseudomonas aeruginosa and is not intended for use in the diagnosis of disease or other conditions in humans. Conforms to Harmonized USP/EP/JP Requirements. A medium recommended by the Harmonized USP/EP/JP for the isolation and identification of Pseudomonas aeruginosa, in non- sterile pharmaceutical samples. Conforms to USP/EP/JP performance specification. Gelatin is a source of nitrogen while glycerol acts as a carbon source. Cetrimide is a quaternary ammonium compound that inhibits the growth of a wide range of Gram-positive and some Gram-negative micro-organisms. Magnesium chloride and Dipotassium sulphate improve the production of pyoverdin and pyocyanin pigments that combine to give Pseudomonas aeruginosa characteristic green colonies. According to the Harmonized USP/EP/JP, subculture is carried out onto the medium after enrichment in Casein Soya Bean Digest Broth.

Typical Formulation

Formula Litre
Pancreatic Digest of Gelatin 20.0 g/L
Magnesium Chloride 1.4 g/L
Dipotassium Sulfate 10.0 g/L
Cetrimide 0.3 g/L
Agar 13.6 g/L

Preparation

Suspend 45.3 g of the medium in one liter of purified water.
Add 10 mL of Clycerol.
Heat with frequent agitation and boil for one minute to completely dissolve the medium.
Autoclave at 121°C for 15 minutes.
Cool to 45–50 °C.

Previous Item Number: This product was previously known as Acumedia Product: 7688 Cetrimide Agar (Agar Medium N); and LabM Product: HP010 Cetrimide Agar (USP/EP/JP)