Baird-Parker Agar, 500g

Baird-Parker Agar is used for the detection and enumeration of Staphylococcus aureus in foods and is not intended for use in the diagnosis of disease or other conditions in humans. Baird-Parker Agar was first described in 1962. It is a selective medium for the isolation and presumptive identification of coagulase-positive staphylococci. This medium is extensively used for detecting Staphylococcus aureus in foods, dairy products, and other materials. Coagulase-positive staphylococci can grow and reproduce in cosmetic products. These products are tested for the presence of coagulase-positive staphylococci using standard microbiological methods.

Typical Formulation

Formula Litre
Enzymatic Digest of Casein 10.0 g/L
Beef Extract 5.0 g/L
Yeast Extract 1.0 g/L
Lithium Chloride 5.0 g/L
Glycine 12.0 g/L
Sodium Pyruvate 10.0 g/L
Agar 17.0 g/L*
*15–20 g according to gel strength

Preparation

Suspend 60 g of the medium in one liter of purified water.
Heat with frequent agitation and boil for one minute to completely dissolve the medium.
Autoclave at 121°C for 15 minutes.
After cooling to 45–50 °C, add 50 mL of Egg Yolk Tellurite Supplement (#7983 or X085). Alternatively, add 50 mL of Egg Yolk Emulsion (#7982) and 10 mL of Tellurite Supplement (1%), (#7989).
Mix thoroughly before dispensing.