Simple way to doble up multiplexing capacity in any real-time PCR Instrument
Enable any existing Real-time PCR instrument to be more powerful system by double up its capability for multiplexing without upgrading the hardware.
1. Conventional SNP genotyping assay requires a single tube and two channels to analyze one SNP target. In other words, four tubes are required to analyze four SNP targets. In order to minimize the number of channels, melting curve analysis after target amplification is necessary. Limited multiplexity and longer turnaround time (TAT) due to melting curve analysis are main hurdles in current SNP genotyping method.
2. MuDT™ – based SNP genotyping assay can provide a SNP genotyping in a single channel, which allows a genotyping of four SNP targets in a single tube.
Identification of multiple mutations simultaneously in a single tubes
MUDT™ allows the detection and quantification of multiple mutations in a single tube.
Single-channel multiplexing without melting curve analysis in real-time PCR Technical Papers
Young-Jo Lee, Daeyoung Kim, Kihoon Lee & Jong-Yoon Chun ｜SCIENTIFIC REPORTS | 4:7439 | DOI: 10.1038/srep07439