1. What is molecular diagnostics?

Molecular Diagnostics is referred to as the detection of genomic variants, aiming to facilitate detection, diagnosis, sub classification, prognosis, and monitoring response to therapy.

Visitors Count: 8178

2. Why is molecular diagnostics important?

Most important, by adopting molecular diagnostics, laboratories are having a greater impact on the treatment and care of patients. These advanced systems are helping physicians treat infectious diseases sooner and with more precision.

Visitors Count: 8178

3. What is molecular testing used for?

In medicine, a laboratory test that checks for certain genes, proteins, or other molecules in a sample of tissue, blood, or other body fluid. Molecular tests also check for certain changes in a gene or chromosome that may cause or affect the chance of developing a specific disease or disorder, such as cancer.

Visitors Count: 8178

4. What are advantages of molecular diagnostics over conventional methods?

With regard to infectious disease, molecular diagnostics has major advantages over the sole use of serology. Molecular diagnostics is a more sensitive method allowing detection of lower amounts of infectious agents and therefore giving the ability to detect infections earlier than was previously possible.

Visitors Count: 8178

5. What is PCR?

Polymerase Chain Reaction, or PCR, is a technique to make many copies of a specific DNA region in vitro (in a test tube rather than an organism). … PCR has many research and practical applicationsIt is routinely used in DNA cloning, medical diagnostics, and forensic analysis of DNA.

Visitors Count: 8178

6. How is PCR used to diagnose?

The use of Polymerase Chain Reaction (PCR) in infectious disease diagnosis, has resulted in an ability to diagnose early and treat appropriately diseases due to fastidious pathogens, determine the antimicrobial susceptibility of slow growing organisms, and ascertain the quantum of infection.

Visitors Count: 8178

7. What is the principle of PCR?

Its principle is based on the use of DNA polymerase which is an in vitro replication of specific DNA sequences. This method can generate tens of billions of copies of a particular DNA fragment (the sequence of interest, DNA of interest, or target DNA) from a DNA extract (DNA template).

Visitors Count: 8178

8. What are the three steps of PCR?

PCR is based on three simple steps required for any DNA synthesis reaction: (1) denaturation of the template into single strands; (2) annealing of primers to each original strand for new strand synthesis; and (3) extension of the new DNA strands from the primers.

Visitors Count: 8178

9. Is real time PCR quantitative?

Quantitative PCR (qPCR), also called realtime PCR or quantitative realtime PCR, is a PCR-based technique that couples amplification of a target DNA sequence with quantification of the concentration of that DNA species in the reaction.

Visitors Count: 8178

10. What instrument is used for PCR?

The thermal cycler (also known as a thermocyclerPCR machine or DNA amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR). E.g CFX96 from biorad (www.biorad.com)

Visitors Count: 8178

11. How does a thermocycler work?

Thermocyclers, or thermal cyclers, are instruments used to amplify DNA and RNA samples by the polymerase chain reaction. The thermocycler raises and lowers the temperature of the samples in a holding block in discrete, pre-programmed steps, allowing for denaturation and reannealing of samples with various reagents.

Visitors Count: 8178

12. What is the function of primers in PCR?

PCR primers are short fragments of single stranded DNA (15-30 nucleotides in length) that are complementary to DNA sequences that flank the target region of interest. The purpose of PCR primers is to provide a “free” 3′-OH group to which the DNA polymerase can add dNTPs.

Visitors Count: 8178

13. How Fast Is PCR?

Most users of the polymerase chain reaction (PCR) would describe it as a fairly fast technique, taking about 45 min to an hour to complete 40 cycles, depending on the particular protocol and instrument used.

Visitors Count: 8178

14. What is the advantage of real time PCR?

One advantage of RealTime PCR over traditional PCR is that it is a closed-tube system requiring no post-PCR processing. RealTime PCR has higher precision, increased sensitivity (down to one copy), increased dynamic range (greater than 8 logs), and high resolution (less than two-fold differences).

Visitors Count: 8178

15. What is the difference between real time PCR and PCR?

Traditional PCR has advanced from detection at the end-point of the reaction to detection while the reaction is occurring. RealTime chemistries allow for the detection of PCR amplification during the early phases of the reaction.

Visitors Count: 8178

16. What are the two primers used in PCR?

Two primers are utilized, one for each of the complementary single strands of DNA released during denaturation. The forward primer attaches to the start codon of the template DNA (the anti-sense strand), while the reverse primer attaches to the stop codon of the complementary strand of DNA (the sense strand).

Visitors Count: 8178

17. What are the 5 steps of PCR?

A For efficient endpoint PCR with fast and reliable results, here are five key steps to consider:

  • Step 1 DNA isolation.
  • Step 2 Primer design.
  • Step 3 Enzyme selection.
  • Step 4 Thermal cycling.
  • Step 5 Amplicon analysis.
Visitors Count: 8178

18. Is Qpcr more sensitive than PCR?

Quantitative Real-Time PCR Is Not More Sensitive than “Conventional” PCR. Molecular methods, essentially based upon PCR, have become an indispensable tool in the diagnosis of infectious diseases.

Visitors Count: 8178

19. What is the difference between Open and Closed Automation Systems?

Open systems:

Open systems are automated systems that allow for the inclusion of hardware, software, and instrumentation from many vendors. One of the major advantages of an open system is that it offers more flexibility for instrumentation choices and allows a greater degree of accommodation for any changes that may be desired over time. It should be noted that initial implementation and planning may be more involved. This is because ALL components of the system from various vendors must be integrated with each other, as well as with the LIS and/or workflow management applications where compatibility issues may be an obstacle.

e.g Hamilton nimbus, tanbead, Hamilton starlet, Biorad CFX96

 

Closed system:

In contrast, closed systems exist as packages of pre-analytic automation, analytic phase instrumentation, and post-analytic systems from a single vendor. The accompanying hardware and software are designed specifically to integrate together with that vendor’s other instrumentation and applications. An appeal of the closed system is this “turnkey” benefit, since closed systems are “ready to go” and can often be up and running more quickly with less planning and implementation efforts by the laboratory. However, the trade off with closed systems is less long-term flexibility, since closed systems can often be more difficult or costly to modify after installation. If instrumentation changes are desired in the future, it may be challenging or impossible for any existing closed system components to be integrated with competing vendor’s hardware, software, or instrumentation.

The choice of either an open or closed system will depend on a particular laboratory’s needs. Great improvement in both process efficiency and specimen identification accuracy is frequently reflected when either an open or closed system is implemented effectively.

e.g. Roche, biomeriuex

Visitors Count: 8178

20. Which facilities are using Seegene technology in the country?

  1. KEMRI: https://www.kemri.org
  2. CGIAR: https://www.cgiar.org
  3. Primate Research: http://primateresearch.org
  4. Lancet Kenya: https://lancet.co.ke
  5. Hercules laboratory: https://hercules-healthcare.business.site
  6. Star Metropolis Lab: http://metropolisafrica.com/
  7. Sonar Laboratories: https://sonar-imaging-centre.business.site/?m=true
  8. Aga Khan Hospital:         https://hospitals.aku.edu/nairobi/Pages/default.aspx
  9. Kenyatta National Hospital: https://knh.or.ke
  10. National Public Health Laboratory Kenya: https://nphl.go.ke
  11. Ministry of Health –Kenya: https://www.health.go.ke
  12. University of Nairobi: https://chs.uonbi.ac.ke
  13. JKUAT:  http://www.jkuat.ac.ke/colleges/cohes/
  14. Kenyatta University: https://kutrrh.go.ke
  15. Nairobi Women’s Hospital – all branches: https://nwh.co.ke/hospital/
  16. Avenue Hospital – All branches:  http://www.avenuehealthcare.com/index.php
  17. Metropolitan Hospital – All branches: https://www.metro-hospital.com
  18. Ladnan Hospital – All branches: https://ladnan.org
Visitors Count: 8178

21. Do you offer equipment Equipment Placement /Leasing?

We offer tailor made solutions that may include placement and leasing for select customers with a view to ramping up sales and technology uptake.

Contact us for further information.

Visitors Count: 8178

22. How does Seegene compare to other Molecular diagnostic companies

Visitors Count: 8178

Questions?

Evaluating our products and need advice before you buy?

Contact us

About Us

Avicalab Diagnostics is one of the leading distributors of diagnostics, scientific and life science research products, laboratory chemicals, reagents and equipment.

Learn More
Visitors Count: 8178